antiSMASH
v7.0.0beta1-67b538a9
Date: May 27, 2023 07:52:56 pm
Annotation id: 10229392
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Emerald
v0.2.4.1
Date: May 27, 2023 07:52:58 pm
Annotation id: 10229577
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CDDSearch
Date: May 27, 2023 07:53:00 pm
Annotation id: 10229731
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InterProScan
Date: May 27, 2023 07:52:58 pm
Annotation id: 10229578
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Comments (3 posts)
SMC Bot May 27, 2023 07:52:56 pm
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This BGC may be derived from merging overlapping BGC calls from multiple genome mining tools. The GFF attributes from the original BGC annotations are shown below in JSON format:
[
{
"SequenceID/Contig": "NZ_LEKG01000004.1",
"start": "1",
"end": "10543",
"annotation_tool": "antiSMASHv7.0.0beta1-67b538a9",
"candidate_cluster_numbers": "1",
"contig_edge": "True",
"product": "RiPP-like",
"region_number": "1",
"rules": "(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or TIGR01193 or (YcaO or TIGR03604))",
"tool": "antismash"
},
{
"SequenceID/Contig": "NZ_LEKG01000004.1",
"start": "139",
"end": "79962",
"annotation_tool": "EMERALDv0.2.4.1",
"ID": "NZ_LEKG01000004.1_emrld_1",
"nearest_MiBIG": "BGC0001330",
"nearest_MiBIG_class": "NRP Polyketide",
"nearest_MiBIG_diceDistance": "0.299",
"score": "0.960",
"partial": "10"
},
{
"SequenceID/Contig": "NZ_LEKG01000004.1",
"start": "11765",
"end": "79772",
"annotation_tool": "antiSMASHv7.0.0beta1-67b538a9",
"candidate_cluster_numbers": "2,3,4,5",
"contig_edge": "False",
"product": "terpene,T1PKS,NRPS-like,NRPS",
"region_number": "2",
"rules": "(Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5),cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)),cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX)),cds(Condensation and (AMP-binding or A-OX))",
"tool": "antismash"
}
]
Thomas Tørring Nov 20, 2023 02:13:14 am
FlaggedHidden
We have confirmed the production of the seven BE-43547 congeners from this strain and the this particular BGC has all of the expected domains and module. The odd looking arrangement of the KR-DH domains in ctg304_30 (normally DH preceeds KR in primary sequence) is something we observed in all BGCs associated with APD-CLD compounds (rakicidins, microtermolide, BE-43547).
Daniel Udwary Nov 23, 2023 05:57:09 pm
FlaggedHidden
Very cool! Thanks for sharing this! I'm pasting in the molecules' structures for reference from your 2016 synthesis paper.
Comments (3 posts)
SMC Bot
May 27, 2023 07:52:56 pm
Flagged Hidden
This BGC may be derived from merging overlapping BGC calls from multiple genome mining tools. The GFF attributes from the original BGC annotations are shown below in JSON format:
Thomas Tørring
Nov 20, 2023 02:13:14 am
Flagged Hidden
We have confirmed the production of the seven BE-43547 congeners from this strain and the this particular BGC has all of the expected domains and module. The odd looking arrangement of the KR-DH domains in ctg304_30 (normally DH preceeds KR in primary sequence) is something we observed in all BGCs associated with APD-CLD compounds (rakicidins, microtermolide, BE-43547).
Daniel Udwary
Nov 23, 2023 05:57:09 pm
Flagged Hidden
Very cool! Thanks for sharing this! I'm pasting in the molecules' structures for reference from your 2016 synthesis paper.